Pcr Protocols

GO Pcr Protocols


Author: Nicola King
Type: eBook
Language: English
Released: 2010
Publisher: Humana Press
Page Count: 519
Format: pdf
ISBN-10: 1607616289
ISBN-13: 9781607616283
Tags:Pcr Protocols, tutorials, pdf, djvu, chm, epub, ebook, book, torrent, downloads, rapidshare, filesonic, hotfile, fileserve


Description:
The Institute for Genomic Research, Rockville, MD. Describes the methods for transferring and maintaining DNA within and between species, presented in step-by-step format. Includes scientific reviews, software tools, database resources, and genome sequencing strategies. DNLM: Chromosomes, Artificial, Bacterial. From the Back Cover The ability to transfer and maintain DNA both within and between species is an essential skill in biotechnology and medicine. In Bacterial Artificial Chromosomes, expert investigators describe not only the classic methods, but also the many novel techniques they have perfected for the transfer of large DNAs into the cells of both microbes and animals via large-insert recombinant DNAs. Volume 2: Functional Studies provides a wide variety of methods and applications for functional analysis of the DNA-transformed organisms. The laboratory protocols follow the successful Methods in Molecular Biologyв„ў series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Besides protocols, each chapter includes scientific reviews, software tools, database resources, genome sequencing strategies, and illustrative case studies. An accompanying volume, Volume 1: Library Construction, Physical Mapping, and Sequencing, presents readily reproducible techniques for library construction, physical mapping, and sequencing. Comprehensive and cutting-edge, the two volumes of Bacterial Artificial Chromosomes provide a superlative collection of highly productive protocols that will prove useful to many bioscientists, including genome sequencers, geneticists, molecular biologists, and biochemists studying the structure and function of specific genomes.